Protein Marker For Native Gel. Load 5 µl PageRuler Prestained Protein Ladder into marker narrow well Fig. Color Marker Ultra-low Range C6210 separated on a 16 Tris-tricine gel. Annons Sign Up Today For News Announcements. Gel used for native non-denaturing gel electrophoresis of protein samples.
Originally molecular weight markers were a mixture of easy-to-purify proteins of known molecular weight. Protein molecular weight markers typically used polyacrylamide gel electrophoresis and Western blotting. NativePAGE Bis-Tris Gels use Coomassie G-250 to bind to proteins and confers a net negative charge while maintaining the proteins in their native state without protein denaturation. Coomassie Brilliant Blue R250 and PAGE Blue 83 each visibly stain as little as 01-1 ug of protein. Color Marker Ultra-low Range C6210 separated on a 16 Tris-tricine gel. Invitrogen Life Technologies have predesigned molecular wt markers for the native PAGE NativeMark Unstained Protein Standard Cat LC075.
The NativePAGE Novex Bis-Tris Gels are used with NativePAGE Running Buffers see page 5 to produce a non-denaturing electrophoresis system operating at near neutral pH.
IEF and 2D protein standards are a mixture of native proteins with isoelectric points pI ranging from 445 to 96 providing reproducible pI calibration in native PAGE or agarose IEF gels. 2-D SDS-PAGE protein standards provide calibrated references for protein pI and molecular weight in the second dimension. IEF and 2D protein standards are a mixture of native proteins with isoelectric points pI ranging from 445 to 96 providing reproducible pI calibration in native PAGE or agarose IEF gels. The G-250 is present in the cathode buffer to provide a continuous flow of G-250 into the gel and is added to samples containing non-ionic detergent prior to loading the samples onto the gel. These marker proteins were unstained and were generally visualized on SDS-PAGE gels by staining the gel with Coomassie Brilliant Blue R250 or after western transfer by a stain such as Ponceau S. Invitrogen Life Technologies have predesigned molecular wt markers for the native PAGE NativeMark Unstained Protein Standard Cat LC075.