Polarity Of Gc Columns. This will separate complex mixtures of chemicals or proteins into their various different components. The columns deliver industry-leading technology with the highest inertness lowest bleed levels and tightest column-to-column reproducibility specifications. Analytes with polar groups. Column temperature A excessively high column temperature results in very short retention time but also in a very poor separation because all components mainly stay in the gas phase.
Luigi Mondello University of Messina Italy. Column temperature A excessively high column temperature results in very short retention time but also in a very poor separation because all components mainly stay in the gas phase. Polar analytes require polar stationary phases to be retained in the sp. Polarity Scale ZebronTM HighlightPhase 5 ZB-1 Non-polar phase suited for true boiling point separations 5 ZB-1ms Extremely low bleed column for non-polar compounds 5 ZB-1HT Inferno High temperature stability 430 C for non-polar compounds 5 ZB-1XT SimDist Metal column with increased accuracy for simulated distillation analysis high temperature. At present it is estimated that more than 80 of all applications are run on capillary columns due to the fast and efficient separation the afford. Limit2 C ApplicationsFeatures Low bleed GC-MS DB-1ms 100 Dimethylpolysiloxane Non-polar 340360 General purpose very low bleed characteristics ideal for GC-MS semivolatiles halogenated compounds pesticides drugs of abuse amines HP-1ms 100 Dimethylpolysiloxane Non-polar 325350.
Non-polar analytes require lessnon-polar stationary phases.
This is the advantage of capillary columns. A wide range of world leading polar stationary phase GC columns for the analysis of highly polar analytes - delivering best in class peak shape and the lowest bleed profiles even for these highly polar phases. The Agilent Polar GC column range includes highly popular phases such as DB Wax and DB-FFAP phases. High Polarity GC Columns from Agilent. Agilent JW PLOT columns begin with the designation of. McReynolds Constants are then calculated using the retention index data of the column relative to the.