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Sep
Lysosomal Activity Assay Kit. Includes necessary substrate product and internal standard all provided at known concentrations. Assay Genies Lysosome Isolation Kit provides a procedure for isolating enriched or purified lysosomal fraction from animal tissues and cultured cells by differential centrifugation followed by density gradient centrifugation. If your compound inhibits lysosomal proteolysis you will see a large accumulation of steady-state LC3-II by western blot. The self-quenched substrate which acts as an endocytic cargo can be taken up by cells and degraded within an endolysosomal vesicle.
Defects in lysosomal enzyme activity have been associated with a variety of diseases including Parkinsons Tay-Sachs Sandhoff Krabbe Wolman and Gaucher syndromes. This assay should give a robust read-out of autophagic-lysosomal flux. Lysosomal Intracellular Activity Assay Kit Cell-Based includes Cytochalasin D a cell-permeable inhibitor of endocytosis that serves as an experimental control. Choose from a wide variety of cell-based assays designed for greater accuracy and increased throughput. The LAL Activity MaxSpec Assay Substrate P-PMHC has been designed to function as a highly selective substrate for LAL. The presence of lysosomes can be determined by measuring the acid phosphatase activity with the Acid Phosphatase Assay Kit Catalog Number CS0740 or the β-N-Acetylglucosaminidase activity with the β-N-Acetylglucosaminidase Assay Kit Catalog Number CS0780.
Lysosomal Intracellular Activity Assay Kit Cell-Based includes Cytochalasin D a cell-permeable inhibitor of endocytosis that serves as an experimental control.
BioVisions Lysosomal Intracellular Activity Assay Kit Cell-Based includes Bafilomycin A1 a cell-permeable inhibitor of the lysosomal membrane V-ATPase proton pump which serves as an anti-lysosomal experimental control. Lysosomal Intracellular Activity Assay Kit Cell-Based. Elucidation of the mechanisms of endocytic pathway in living cells. BioVisions Lysosomal Intracellular Activity Assay Kit Cell-Based includes Bafilomycin A1 a cell-permeable inhibitor of the lysosomal membrane V-ATPase proton pump which serves as an anti-lysosomal experimental control. Simple assay to measure intracellular lysosomal activity as well as to screenstudycharacterize lysosomal inhibitors. Lysosomal Intracellular Activity Assay Kit Cell-Based includes Cytochalasin D a cell-permeable inhibitor of endocytosis that serves as an experimental control.