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Glycogen Phosphorylase Activity Assay. A new assay for glycogen phosphorylase EC 2411 is presented. The OD 450 nm signal is directly proportional to the glycogen phosphorylase activity. The kit can detect as low as 10 mU in a variety of sample types. The OD 450 nm signal is directly proportional to the glycogen phosphorylase activity.
Characterization of the substrate specificity of GP using. It should find utility for assay. This method is good enough for the samples with high phosphorylase activities but not for the ones with low activities. The kit is a rapid sensitive and convenient tool for detecting glycogen. The method could be widely employed for the assay of phosphate-releasing reaction from labile organic phosphates such as for the assay of glycogen phosphorylase. The kit can detect as low as 10 mU in a variety of sample types.
37 Full PDFs related to this paper.
Hepatic Glycogen Synthesis Is Highly Sensitive to Phosphorylase Activity. PromoCells Glycogen Phosphorylase Activity Assay Kit is based on the detection of G1P by a set of enzymatic reactions to generate a colored product with a strong absorbance at 450 nm. 37 Full PDFs related to this paper. Journal of Biological Chemistry 2001. The action of glycogen phosphorylase GP is essentially reversible although GP is generally classified as a glycogen-degrading enzyme. Activity phosphorylase has a very high greater than unity negative control coefficient on glycogen synthe-sis regardless of whether it is determined by enzyme inactivation or overexpression.